Automated Workflows for Luminex xMAP Assays

Bead-based, multiplexing assays such as those provided by the Luminex xMAP technology, have proven to be highly useful for biomarker identification and quantification from cells, tissues and body fluids. Applications include biological research, drug discovery and clinical diagnostics. The original technology based on the use of polystyrene microspheres tends to have arduous, manual work flows prone to cumulative systematic and random error that can lead to problematic precision for quantification. Here we demonstrate improvements in precision and ease-of-use through the use of BioTek microplate washers for both polystyrene and magnetic microspheres. Automation of workflows for partially-full microplates to full 96and 384-well microplates can be performed.